A defined minimal medium for axenic strains of Dictyostelium discoideum.
نویسندگان
چکیده
A defined, minimal medium for the cellular slime mold Dictyostelium discoideum is reported.
منابع مشابه
Relationship between axenic growth of Dictyostelium discoideum strains and their track morphology on substrates coated with gold particles
Amoebae of Dictyostelium discoideum produce tracks with two distinct morphologies on gold-coated coverslips. The wild-type strain and other strains that feed only by phagocytosis produced indistinct, fuzzy tracks, whereas mutants capable of axenic growth produced clear, sharp tracks. The sharp track morphology was found to be a recessive phenotype that segregates with axenicity and probably req...
متن کاملGrowth of myxameobae of the cellular slime mould Dictyostelium discoideum in axenic culture.
1. A simple axenic medium suitable for the growth of the myxamoebae of a strain of the cellular slime mould Dictyostelium discoideum is described. 2. Procedures suitable for the growth of this strain in liquid and on solid media are described. 3. Conditions suitable for initiating the cell differentiation of myxamoebae grown axenically are described.
متن کاملObservations on the growth of Dictyostelium discoideum in axenic medium: evidence for an extracellular growth inhibitor synthesized by stationary phase cells.
By analysing the growth kinetics of Dictyostelium discoideum amoebae in varying combinations of the components of the nutrient medium HL-5 (Cocucci & Sussman, 1970), we have demonstrated that Dictyostelium amoebae enter stationary phase when they deplete the medium of one or more nutrient components. However, if the medium of a stationary phase culture is supplemented with any of the initial co...
متن کاملDiscoidin-binding polysaccharide from Dictyostelium discoideum.
Extracts of Dictyostelium discoideum grown axenically in a chemically defined medium were evaluated for binding to discoidin I and discoidin II, endogenous lectins of this slime mold. Binding activity was measured by competitive inhibition of 125I-lactosyl-bovine serum albumin binding to the immobilized lectins. With the solubilization procedure used extracts of vegetative cells and of early ag...
متن کاملRelevant Genes Linked to Virulence Are Required for Salmonella Typhimurium to Survive Intracellularly in the Social Amoeba Dictyostelium discoideum
The social amoeba Dictyostelium discoideum has proven to be a useful model for studying relevant aspects of the host-pathogen interaction. In this work, D. discoideum was used as a model to study the ability of Salmonella Typhimurium to survive in amoebae and to evaluate the contribution of selected genes in this process. To do this, we performed infection assays using axenic cultures of D. dis...
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ورودعنوان ژورنال:
- Proceedings of the National Academy of Sciences of the United States of America
دوره 74 5 شماره
صفحات -
تاریخ انتشار 1977